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All main functions of an organism, such as the structure and appearance, are determined by the genetic material, i.e. the DNA. The complete set of information in the DNA of an organism is called the genome. The regions that encode the genetic information are called genes.
Today it is possible to study ancient DNA, i.e. any amount of genetic material that can be recovered from dead organisms (skeletons, mummies, remains of prehistoric or extinct animals) found in archaeological excavations. With the help of specialised laboratory techniques, the evolution of ancient human and animal populations can be reconstructed.
Given that the ancient DNA is found in extremely small quantities and can be contaminated by present-day DNA, its analyses are conducted in specialised laboratories.
Researchers wear gloves, masks and coverall lab suits.
Sampling involves the removal of a small bone fragment by a specialised researcher.
The sample is mechanically cleaned and placed under UV radiation lamps, which destroy the DNA that lies on its surface. Then it is ground to fine powder.
The DNA must be isolated from the other cellular components. This is why it is added into a chemical solution and after a series of lab analyses, the DNA molecules are collected.
The quantity of the resulting isolated DNA is too small for the necessary analyses. This is why researchers try to create thousands of identical copies, by amplifying a specific region of the DNA, which is called target DNA.
These techniques are extremely demanding and the sample may be contaminated by even a single present-day DNA molecule in the lab. Therefore, it is fundamental that the facilities are clean. Every activity in the lab is slow and well organised.
After researchers have ensured there is DNA in the sample, they read the sequence of its bases. This is done through sequencing in “Next-Generation” automatic analysers. Nowadays, it is possible to simultaneously sequence hundreds of sequences (reads) and not just one target DNA.
The final stage features the verification and study of the results. The verification involves authenticity tests. These include tests with mathematical algorithms. Then the hundreds of thousands of base sequences are read.